Carbohydrate analysis and carbohydrate-protein binding studies by capillary/microchip electrophoresis.
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چکیده
منابع مشابه
Studies of carbohydrate-protein interaction by capillary electrophoresis.
T he high cap abilities of cap i l l a ry electro p h o resis in both separation and detection have been well recognized. It allows high resolution separation with theoretical plate numbers often exceeding several hundred thousands and rep ro d u c i ble quantifi c ation by on-cap i l l a ry detection. In addition to these adva n t age s , c ap i l l a ry electrophoresis has a characteristic fe...
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The binding of carbohydrates to the hemelytic lectin CEL-III isolated from the marine inyertebrate dacumaria echinata was studied. Equilibrium dialysis data s"ggest that CEL-III has two carbohydrate-binding sites with equal aMnity. The binding of specific carbohydrates to CEL-III i"duces a decrease in the fluorescence intensity at 339 nm and the shift of the fluorescence emission maximum to a w...
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The interactions of trimannosides 1 and 2 with Con A were studied to reveal the effects of displacement of well-ordered water molecules on the thermodynamic parameters of protein-ligand complexation. Trisaccharide 2 is a derivative of 1, in which the hydroxyl at C-2 of the central mannose unit is replaced by a hydroxyethyl moiety. Upon binding, this moiety displaces a conserved water molecule p...
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Aldonate coupling, a new, high-yield method for conjugation of reducing oligosaccharides to proteins is described. It involves oxidation of the oligosaccharides to their corresponding aldonic acids followed by coupling to amino functions of proteins by activation of carboxyl groups with a water-soluble carbodiimide derivative. The immunochemical properties of some of these synthetic glycoprotei...
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The analysis of the fine structure of starches is important to the investigation of linkages between starch structure and function and to the investigation of the properties and roles of starch biosynthetic, modifying and degradation enzymes. Fluorophore-assisted carbohydrate electrophoresis has recently been introduced as a method for the analysis of the oligosaccharide populations released by...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 2002
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.46.63